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Following sonication, samples were centrifuged (12000 g , 15 min, 10 C) and 200 l of the supernatant was transferred to LCMS vials for the subsequent analysis
Collectively, these data highlight combined sertraline/chloroquine treatment as a novel targeted therapy for ser/gly-producing GBMs under hypoxia and low glucose levels, i.e., conditions that reflect the physiological aspects of the GBM microenvironment
Activated charcoal may also interact with NAC, although the interaction is considered moderate
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Methylene blue is administered orally or intravenously depending on the protocol and clinical goals