A peptide hormone and its receptor protein kinase regulate plant cell expansion
Laursen JB, Somers M, Kurz S, et al
Because each sequence in the initial alignment has a length of 30 amino acids and each position can be mutated to one of 19 amino acids (20 if the position is gapped), this gives 570 single-step mutants in the first generation for each sequence in the training set, that is, 71,304 sequences, reducing to 69,639 sequences after removing duplicates
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For balance between cohorts (semaglutide users and non-users), 1:2 nearest-neighbor propensity score matching was applied to account for demographic factors, contraindications for semaglutide, indications for semaglutide use, and use of NAION-associated drugs